Mai Shiokawa Yui Morita Makoto Nagai Makoto Haritani Hiroshi Aoki
Archives of virology 168(12) 294-294 2023年11月 [査読有り]
Congenital tremor (CT) in piglets was first reported in 1922, and although the causative pathogen was unknown for many years, atypical porcine pestivirus (APPV) was recently shown to be the cause. APPV is difficult to isolate, and there have been ...
The Journal of veterinary medical science 2023年1月 [査読有り]
Classical swine fever (CSF) live vaccine used in Japan, GPE- strain, is produced using guinea pig kidney (GPK)-derived primary culture cells. This means that a large number of guinea pigs are used to generate the primary GPK cells needed to produc...
Mami Oba Shoichi Sakaguchi Hong Wu Yoshihiko Fujioka Hitoshi Takemae Hisako Oki Megumi Kawai Mai Shiokawa Hiroshi Aoki Yuka Fukase Hiroo Madarame Takashi Nakano Tetsuya Mizutani Makoto Nagai
The Journal of general virology 103(2) 2022年2月 [査読有り]
A novel picornavirus was isolated from the faeces of a diarrhoeic cow using MA-104 cells at the third blind passage. This virus, named Den1/2021/JPN, was completely sequenced using total RNA from the cell culture supernatant by deep sequencing. Th...
Bovine-derived cultured cells, including Madin-Darby bovine kidney cells, are used worldwide; however, lipofection tend to result in low transfection efficiency, which has impeded the progress of veterinary research. We performed experiments to co...
Transboundary and emerging diseases 69(4) 1761-1769 2021年5月 [査読有り]
Atypical porcine pestivirus (APPV), which has been confirmed to be associated with congenital tremor (CT) in pigs, is a newly discovered porcine virus that has been found in the Americas, Europe and Asia; however, no report of APPV in Japan has be...
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