BIOTECHNOLOGY AND BIOENGINEERING 114(10) 2279-2288 2017年10月 [査読有り]
Cell detachment is an essential process in adherent cell culture. However, trypsinization, which is the most popular detachment technique used in culture, damages cellular membranes. Reducing cellular membrane damage during detachment should impro...
Shogo Iwamoto   Yuta Kasahara   Yayoi Yoshimura   Akira Seko   Yoichi Takeda   Yukishige Ito   Kiichiro Totani   Ichiro Matsuo   
CHEMBIOCHEM 18(14) 1376-1378 2017年7月 [査読有り]
In order for facilitating the synthesis of oligosaccharides, transglycosylation reactions mediated by glycoside hydrolases have been studied in various contexts. In this study, we examined the transglycosylating activity of a Golgi endo-alpha-mann...
Taiki Kuribara   Makoto Hirano   Gaetano Speciale   Spencer J. Williams   Yukishige Ito   Kiichiro Totani   
CHEMBIOCHEM 18(11) 1027-1035 2017年6月 [査読有り]
Within the endoplasmic reticulum, immature glycoproteins are sorted into secretion and degradation pathways through the sequential trimming of mannose residues from Man(9)GlcNAc(2) to Man(5)GlcNAc(2) by the combined actions of assorted -1,2-mannos...
Kiichiro Totani   Kenta Yamaya   Makoto Hirano   Yukishige Ito   
CARBOHYDRATE RESEARCH 439 16-22 2017年2月 [査読有り]
Glycoprotein N-linked oligosaccharides in the endoplasmic reticulum function as tags to regulate glycoprotein folding, sorting, secretion and degradation. Since the N-glycan structure of a glycoprotein should reflect the folding state, N-glycan pr...