Toshihiro Kasama

This study introduces an automated enzyme-linked immunosorbent assay (ELISA) system employing a novel dip methodology. Unlike traditional ELISA, which requires complex liquid handling, this approach keeps samples and reagents stationary while immersing antibody-coated reaction fields sequentially. This design eliminates pumps and tubing, reducing maintenance, reagent waste, and overall costs. The system, controlled via a microcontroller and web-based interface, demonstrated accurate and efficient C-reactive protein analysis in human serum. With reduced reagent volumes, lower operational costs, and simplified handling, this system offers significant potential for point-of-care testing, telemedicine, and broader next-generation healthcare applications.