We report a novel in vitro high-throughput (HTP) kinase assay using surface plasmon resonance (SPR). In vitro tyrosine phosphorylation was performed in a microtiter plate, after which the substrate was captured with an antibody on a sensor chip an...
H Izuta M Ikeno N Suzuki T Tomonaga N Nozaki C Obuse Y Kisu N Goshima F Nomura N Nomura K Yoda
GENES TO CELLS 11(6) 673-684 2006年6月 [査読有り]
The centromere is a chromatin structure essential for correct segregation of sister chromatids, and defects in this region often lead to aneuploidy and cancer. We have previously reported purification of the interphase centromere complex (ICEN) fr...
K Ikeda H Nakazawa A Shimo-Oka K Ishio S Miyata Y Hosokawa S Matsumura H Masuhara S Belloncik R Alain N Goshima N Nomura K Morigaki A Kawai T Kuroita B Kawakami Y Endo H Mori
PROTEOMICS 6(1) 54-66 2006年1月 [査読有り]
Cypoviruses are insect viruses that produce a cytoplasmic crystalline particle called the polyhedron in which progeny virions are occluded. The virion structural protein, VP3, is implicated in the occlusion of viral particles into polyhedra. In th...
Jun-ichi Takeda Yutaka Suzuki Mitsuteru Nakao Roberto A. Barrero Kanako O. Koyanagi Lihua Jin Chie Motono Hiroko Hata Takao Isogai Keiichi Nagai Tetsuji Otsuki Vladimir Kuryshev Masafumi Shionyu Kei Yura Mitiko Go Jean Thierry-Mieg Danielle Thierry-Mieg Stefan Wiemann Nobuo Nomura Sumio Sugano Takashi Gojobori Tadashi Imanishi
NUCLEIC ACIDS RESEARCH 34(14) 3917-3928 2006年 [査読有り]
We report the first genome-wide identification and characterization of alternative splicing in human gene transcripts based on analysis of the full-length cDNAs. Applying both manual and computational analyses for 56 419 completely sequenced and p...
C Yamasaki KO Koyanagi Y Fujii T Itoh R Barrero T Tamura Y Yamaguchi-Kabata M Tanino J Takeda S Fukuchi S Miyazaki N Nomura S Sugano T Imanishi T Gojobori
GENE 364 99-107 2005年12月 [査読有り]
H-Invitational Database (H-InvDB; http://www.h-invitational.jp/) is a human transcriptome database, containing integrative annotation of 41,118 full-length cDNA clones originated from 21,037 loci. H-InvDB is a product of the H-Invitational project...
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