Tatsuro Shibui   Hiroyoshi Hara   
BIOTECHNOLOGY PROGRESS 33(5) 1201-1208 2017年9月 [査読有り]
Pichia pastoris has been used for the production of many recombinant proteins, and many useful mutant strains have been created. However, the efficiency of mutant isolation by gene-targeting is usually low and the procedure is difficult for those ...