CZECH JOURNAL OF ANIMAL SCIENCE 55(2) 49-57 2010年2月 [査読有り]
Conflicting data still exist regarding the extent of paternal pronuclear DNA demethylation in one cell-stage mammalian embryos. Demethylation of paternal pronuclear DNA was observed in in vivo produced porcine zygotes, whereas in vitro produced em...
Konosuke Okada   Chiara Palmieri   Leonardo Della Salda   Irena Vackova   
ZYGOTE 16(1) 49-56 2008年2月 [査読有り]
The positive effect of strontium ions (Sr(2+)) on sperm motility, capacitation and acrosome reaction has been demonstrated in the mouse, human, guinea pig and hamster. In the present study, we have evaluated the effect of Sr(2+) on the viability a...
HISTOCHEMISTRY AND CELL BIOLOGY 126(2) 213-217 2006年8月 [査読有り]
DNA methylation/demethylation pattern, determined by 5-methylcytosine (5-MeC) immunostaining, was evaluated in porcine "in vivo" produced embryos from zygote up to the blastocyst stage. In one-cell stage embryos, only the maternal pronucleus showe...
Chromosome separation in meiosis I is different from those in mitosis and meiosis II in that homologs separate from each other in the former while sisters do so in the latter. We show here that meiosis-specific cohesin subunit Rec8 in mouse oocyte...
K Okada   Krylov, V   R Kren   J Fulka   
JOURNAL OF REPRODUCTION AND DEVELOPMENT 52(1) 91-98 2006年2月 [査読有り]
The aim of present study was to optimize culture conditions for pig embryos. Initially, we evaluated three different basic culture conditions. When embryos from electro-activation (parthenotes) or in vitro fertilization (IVF-embryos) were cultured...