Mami Oba Shoichi Sakaguchi Hong Wu Yoshihiko Fujioka Hitoshi Takemae Hisako Oki Megumi Kawai Mai Shiokawa Hiroshi Aoki Yuka Fukase Hiroo Madarame Takashi Nakano Tetsuya Mizutani Makoto Nagai
The Journal of general virology 103(2) 2022年2月
A novel picornavirus was isolated from the faeces of a diarrhoeic cow using MA-104 cells at the third blind passage. This virus, named Den1/2021/JPN, was completely sequenced using total RNA from the cell culture supernatant by deep sequencing. Th...
Mai Shiokawa Ryotaro Miura Aki Okubo Yujiro Hagita Itaru Yoshimura Hiroshi Aoki
Scientific reports 11(1) 16207-16207 2021年8月
Bovine-derived cultured cells, including Madin-Darby bovine kidney cells, are used worldwide; however, lipofection tend to result in low transfection efficiency, which has impeded the progress of veterinary research. We performed experiments to co...
Atypical porcine pestivirus (APPV), which has been confirmed to be associated with congenital tremor (CT) in pigs, is a newly discovered porcine virus that has been found in the Americas, Europe and Asia; however, no report of APPV in Japan has be...
BACKGROUND: Bovine papillomavirus types 1 and 2 (BPV1/2) infection in horses has been associated with the development of equine sarcoids. Previous findings revealed the presence of sarcoid-associated BPV sequence variants that have been proposed a...
The Journal of veterinary medical science 82(2) 217-223 2020年2月
The etiology of Porcine respiratory disease complex is complicated by infections with multiple pathogens, and multiple infections increase the difficulty in identifying the causal pathogen. In this present study, we developed a detection system of...
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