Pestalotiopsis spp. strains were isolated from leaves of 17 out of 23 plant species. Some of the Pestalotiopsisspp. isolates showed catechol-xylosylation activity. A catechol-xylosylating enzyme was purified from the culturefiltrate of an isolated...
Carbohydrate Research 345(13) 1901-1908 2010年9月 [査読有り]
β-d-Galactopyranosyl-(1→3)-2-acetamido-2-deoxy-d-glucose (LNB) and β-d-galactopyranosyl-(1→3)-2-acetamido-2-deoxy-d-galactose (GNB) decompose rapidly upon heating into d-galactose and mono-dehydrated derivatives of the corresponding 2-acetamido-2-...
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 74(1) 75-81 2010年1月 [査読有り]
Malic enzyme (ME) was purified as an electrophoretically homogenous protein from Rhodopseudomonas palustris No. 7. The molecular weight of ME was estimated to be 650 kDa and that of its subunit, 86 kDa. ME activity was remarkably enhanced by di- a...
Bioscience, Biotechnology, and Biochemistry 73(5) 1123-1128 2009年5月 [査読有り]
We have purified and characterized an oligoxylosyl transfer enzyme (OxtA) from Bacillus sp. strain KT12. In the present study, a N-terminally His-tagged recombinant form of the enzyme, OxtA(H)E, was overproduced in Escherichia coli and applied to ...