30th Annual Congress of the European Society of Mycobacteriology, Best Poster,Rapid detection of respiratory active Mycobacteria by Auramine O-CTC double staining
JOURNAL OF MICROBIOLOGICAL METHODS 82(3) 327-329 2010年9月 [査読有り]
A new method for rapid enumeration of physiologically active mycobacteria was developed by acid-fast bacilli staining (Auramine O) following formazan reduction. Results can be obtained within 90 min by the optimized procedure, while more than one ...
Tomoaki Ichijo   Nobuyasu Yamaguchi   Katsuji Tani   Masao Nasu   
JOURNAL OF HEALTH SCIENCE 56(3) 321-325 2010年6月 [査読有り]
Waterborne diseases were globally occurred, and many people were suffered from and often killed by them. In order to prevent outbreaks of waterborne diseases, rapid detection of pathogenic microbes in aquatic environment is the important strategy ...
JOURNAL OF HEALTH SCIENCE 55(5) 726-731 2009年10月 [査読有り]
Staphylococcus epidermidis (S. epidermidis) often cause sepsis and related diseases by transfusion of contaminated platelet concentrates (PCs). The proliferation process of this bacterium in PCs has been unclear, thus, bio-imaging system was appli...
Tomoaki Ichijo   Nobuyasu Yamaguchi   Katsuji Tani   Masao Nasu   
JOURNAL OF HEALTH SCIENCE 54(1) 123-128 2008年2月 [査読有り]
We applied transcription in vitro following multiplex PCR in order to improve the sensitivity and rapidity of microbial detection in aquatic environment with an oligonucleotide microarray. Transcripts of 16S rRNA gene were fluorescently labeled an...