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研究者業績

Researcher List >> Yoshiki Higuchi
 

Yoshiki Higuchi

 
Avatar
NameYoshiki Higuchi
URLhttp://www.sci.u-hyogo.ac.jp/life/biophys1/index-j.html
AffiliationUniversity of Hyogo
SectionGraduate School of Science
Job titleSpecially Appointed Professor of University of Hyogo
DegreeDoctor of Science(Osaka University)
J-Global ID200901089463368472

Research Interests

 
hydrogenase ,構造生物学 ,タンパク質結晶学 ,Structural Biology ,Protein Crystallography

Research Areas

 
  • Life sciences / Molecular biology / 
  • Life sciences / Biophysics / 
  • Life sciences / Structural biochemistry / 
  • Nanotechnology/Materials / Biochemistry / 

Committee Memberships

 
Jan 2012
 - 
Today
The Protein Society  Editorial Advisory Board of Protein Science
 
2003
 - 
Today
The Japanese Biochemical Society  Councillor
 
Apr 2010
 - 
Mar 2023
The Crystallographic Society of Japan  Councillor
 
Apr 1996
 - 
Mar 1998
The Biophysical society of Japan  committee member of the editorial board
 
Apr 1994
 - 
Mar 1996
The Crystallographic Society of Japan  committee member of the editorial board
 

Awards

 
Dec 2022
Hyogo Prefecture, Hyogo Prefecture Education Achievement Award (Governor Commendation),university education
Yoshiki Higuchi 
 
Apr 2020
Ministry of Education, Culture, Sports, Science and Technology, Commendation for Science and Technology by the Minister of Education, Culture, Sports, Science and Technology,Structural basis of the mechanism of hydrogen metabolism for hydrogenases
Yoshiki Higuchi 
 
Nov 2017
Hyogo prefecture, Hyogo Preecture Science Award,Structural studies on hydrogenases
HIGUCHI Yoshiki 
 
Oct 2014
Iue Memorial Foundation, 38th Iue Culture Prize,X-ray structural study and application for the proteins
HIGUCHI Yoshiki 
 
Nov 1999
The Crystallographic Society of Japan, 1999 Award of Japan Crystallographic Society,X-ray Structure Analysis of Hydrogenase
Yoshiki Higuchi 
 

Papers

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Structure and function relationship of formate dehydrogenases: an overview of recent progress
Ami Kobayashi   Midori Taketa   Keisei Sowa   Kenji Kano   Yoshiki Higuchi   Hideaki Ogata   
IUCrJ   10(5) 544-554   Sep 2023   [Refereed][Invited]
Formate dehydrogenases (FDHs) catalyze the two-electron oxidation of formate to carbon dioxide. FDHs can be divided into several groups depending on their subunit composition and active-site metal ions. Metal-dependent (Mo- or W-containing) FDHs f...
 
New insights into the oxidation process from neutron and X-ray crystal structures of an O2-sensitive [NiFe]-hydrogenase
Takeshi Hiromoto   Koji Nishikawa   Seiya Inoue   Hideaki Ogata   Yuta Hori   Katsuhiro Kusaka   Yu Hirano   Kazuo Kurihara   Yasuteru Shigeta   Taro Tamada   Yoshiki Higuchi   
Chemical Science   35(14) 9306-9315   Aug 2023   [Refereed]
We report the first neutron structure of [NiFe]-hydrogenase in its oxidized state. This study leads to new insights into the oxidized active site and visualization of the protons characteristic of the oxidized enzyme.
 
X‐ray crystallographic and mutational analysis of the <scp>NylC</scp> precursor: Catalytic mechanism of autocleavage and substrate hydrolysis of nylon hydrolase
Seiji Negoro   Naoki Shibata   Dai‐ichiro Kato   Yusuke Tanaka   Kengo Yasuhira   Keisuke Nagai   Shohei Oshima   Yoko Furuno   Risa Yokoyama   Kaito Miyazaki   Masahiro Takeo   Kowit Hengphasatporn   Yasuteru Shigeta   Young‐Ho Lee   Yoshiki Higuchi   
The FEBS Journal      Feb 2023   [Refereed]
 
Reversible Glutamate Coordination to High-Valent Nickel Protects the Active Site of a [NiFe] Hydrogenase from Oxygen
Catharina J. Kulka-Peschke   Anne-Christine Schulz   Christian Lorent   Yvonne Rippers   Stefan Wahlefeld   Janina Preissler   Claudia Schulz   Charlotte Wiemann   Cornelius C. M. Bernitzky   Chara Karafoulidi-Retsou   Solomon L. D. Wrathall   Barbara Procacci   Hiroaki Matsuura   Gregory M. Greetham   Christian Teutloff   Lars Lauterbach   Yoshiki Higuchi   Masaharu Ishii   Neil T. Hunt   Oliver Lenz   Ingo Zebger   Marius Horch   
Journal of the American Chemical Society   144(37) 17022-17032   Sep 2022   [Refereed]
 
Structural Insights into the Very Low Activity of the Homocoenzyme B 12 Adenosylmethylcobalamin in Coenzyme B 12 ‐Dependent Diol Dehydratase and Ethanolamine Ammonia‐Lyase
Naoki Shibata   Yoshiki Higuchi   Bernhard Kräutler   Tetsuo Toraya   
Chemistry – A European Journal   e202202196(65) e202202196   Sep 2022   [Refereed]
The X-ray structures of coenzyme B12 (AdoCbl)-dependent eliminating isomerases complexed with adenosylmethylcobalamin (AdoMeCbl) have been determined. As judged from geometries, the Co-C bond in diol dehydratase (DD) is not activated even in the p...

Misc.

<23456>
 
 
Crystal structure of O2-tolerant [NiFe] hydrogenase reveals the mechanism of O2-tolerance attributable to a redox-dependent conformational change of [4Fe-3S] cluster
SHOMURA Yasuhito   HIGUCHI Yoshiki   
SPring-8 Research Frontier 2011      Jul 2012   [Refereed][Invited]
 
X-ray Structural Study of the Oxygen-tolerant Membrane-bound [NiFe]-hydrogenase -A novel Bifunctional [4Fe-3S] Cluster
SHOMURA Yasuhito   HIGUCHI Yoshiki   
Spring-8/SACLA INFORMATION   17(2) 105-110   Apr 2012   [Refereed][Invited]
 
Structural Chemistry of O2-tolerant Hydrogenase -The Mechanism to Protect the Activity of Enzyme-
Y. Shomura   Y. Higuchi   
Fuel Cell   11(4) 43-48   Apr 2012   [Invited]
 
O2-tolerance of Hydrogenase
Y. SHomura   Y. Higuchi   
Chemitry   67 70-71   Apr 2012   [Invited]
 
新規の鉄-硫黄クラスターが生み出すヒドロゲナーゼの酸素耐性機構
庄村康人   樋口芳樹   
ライフサイエンス新着レビュー (2011)      Oct 2011   [Invited]

Books and Other Publications

12345>
 
 
Yuta Hori, Koji Nishikawa, Yasuteru Shigeta, Yoshiki Higuchi(Part:Contributor, Biological Enzymes and Hydrogenases)
28 Mar 2023   
 
古川良明, 神戸大朋, 城宜嗣, 津本浩平(Part:Joint author, 水素酸化還元酵素ヒドロゲナーゼ)
エヌ・ティー・エス   20 Jan 2021   (ISBN:9784860437060)
 
樋口芳樹(Part:Contributor, 生物のエネルギー代謝酵素の分子進化)
羊土社 実験医学増刊   Mar 2020   (ISBN:9784758103855)
 
樋口芳樹(Part:Contributor, 電子密度分布は構造の全てを語る)
化学同人   Jul 2014   (ISBN:9784759815153)   
 
樋口芳樹(生物による水素エネルギー代謝システム)
羊土社 実験医学増刊   Jun 2014   (ISBN:9784758103398)

Presentations

12345>
 
 
Yoshiki Higuchi   
3rd International Solar Fuels Conference (ISF-3) and International Conference on Artificial Photosynthesis-2019 (ICARP2019)   21 Nov 2019   [Invited]
 
Yoshiki Higuchi   
12th International Hydrogenase Conference 2019   1 Apr 2019   [Invited]
 
Yoshiki Higuchi   
I2CNER International Workshop, Catalysis for Future Energy Technologies 2019   31 Jan 2019   [Invited]
 
Yoshiki Higuchi   
3rd International Symposium and Workshop on Functional Genomic and Structural Biology (FGSB2018)   23 Jul 2018   [Invited]
 
Yoshiki Higuchi   
8th Asian Biological Inorganic Chemistry Conference (AsBIC8)   6 Dec 2016   [Invited]

Teaching Experience

12>
 
Apr 2004
 - 
Mar 2022
Information Science (Faculty of Science, University of Hyogo)
Apr 2002
 - 
Mar 2022
Crystal Structure Analysis of Biopolymers (Department of Picobiology, University of Hyogo)
Apr 2002
 - 
Mar 2022
Information literacy (Faculty of Science, University of Hyogo)
Apr 2002
 - 
Mar 2022
Structural Biology 1 (Department of Science, University of Hyogo)
Apr 2002
 - 
Mar 2004
Biophysics (Faculty of Science, University of Hyogo)

Professional Memberships

12>
 
Apr 2000
 - 
Today
Protein science Society of Japan
Sep 1994
 - 
Today
The Biophysical Society of Japan
Dec 1988
 - 
Today
Tha Japanese Society for Synchrotron Radiation Research
Apr 1987
 - 
Today
The japanese Biochemical Society
Jan 1980
 - 
Today
The Chemical Society Of Japan

Research Projects

12345>
 
 
Structural basis of reaction mechanism and proton pathway of [NiFe]-hydrogenase
Japan Society for the Promotion of Science: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)
Yoshiki Higuchi 
Project Year: Apr 2019 - Mar 2024
 
high speed and local transfer hydrogen and electrons
Japan Society for the Promotion of Science: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
Hatsumi Mori Genki Kobayashi Yoshiki Higuchi 
Project Year: Jun 2018 - Mar 2023
 
Reaction mechanism of [FeFe] hydrogenases
Japan Society for the Promotion of Science: Grants-in-Aid for Scientific Research
Ogata Hideaki Higuchi Yoshiki 
Project Year: 2017 - 2019
 
Activity inhibition mechanism of [NiFe] hydrogenase by oxidative modification of active site cysteine ligand
Japan Society for the Promotion of Science: Grants-in-Aid for Scientific Research
Tai Hulin HIROTA Shun HIGUCHI Yoshiki XU Liyang 
Project Year: Apr 2016 - Mar 2018
 
Structural Basis of Biological System in Hydrogen Energy Metabolism
Japan Science and Technology Agency: Core Research for Evolutional Science and Technology (CREST)
HIGUCHI Yoshiki 
Project Year: Oct 2012 - Mar 2018
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