Hulin Tai Koji Nishikawa Yoshiki Higuchi Zong-Wan Mao Shun Hirota
Angewandte Chemie (International ed. in English) 58(38) 13285-13290 2019年9月 [査読有り]
A [NiFe] hydrogenase (H2 ase) is a proton-coupled electron transfer enzyme that catalyses reversible H2 oxidation; however, its fundamental proton transfer pathway remains unknown. Herein, we observed the protonation of Cys546-SH and Glu34-COOH ne...
Protein science : a publication of the Protein Society 28(3) 663-670 2019年3月 [査読有り]
Enzyme activity is typically assayed by quantitatively measuring the initial and final concentrations of the substrates and/or products over a defined time period. For enzymatic reactions involving gaseous substrates, the substrate concentrations ...
Chemistry (Weinheim an der Bergstrasse, Germany) 24(68) 18052-18058 2018年12月 [査読有り]
Bilirubin oxidase (BOD) belongs to the family of blue multicopper oxidases, and catalyzes the concomitant oxidation of bilirubin to biliverdin and the reduction of molecular oxygen to water via a four-electron reduction system. The active sites of...
Noor Dina Muhd Noor Hiroaki Matsuura Koji Nishikawa Hulin Tai Shun Hirota Jaehyun Kim Jiyoung Kang Masaru Tateno Ki-Seok Yoon Seiji Ogo Shintaro Kubota Yasuhito Shomura Yoshiki Higuchi
Chemical communications (Cambridge, England) 54(87) 12385-12388 2018年10月 [査読有り]
Citrobacter sp. S-77 [NiFe]-hydrogenase harbors a standard [4Fe-4S] cluster proximal to the Ni-Fe active site. The presence of relocatable water molecules and a flexible aspartate enables the [4Fe-4S] to display redox-dependent conformational chan...
Standard [NiFe]-hydrogenase from Desulfovibrio vulgaris Miyazaki F (DvMF-H2ase) catalyzes the uptake and production of hydrogen (H2) and is a promising biocatalyst for future energy devices. However, DvMF-H2ase experiences oxidative inactivation u...
大学本部
理学研究科