Koji Nishikawa Satoko Mochida Takeshi Hiromoto Naoki Shibata Yoshiki Higuchi
Journal of inorganic biochemistry 177 435-437 2017年12月 [査読有り]
Hydrogenase is a key enzyme for a coming hydrogen energy society, because it has strong catalytic activities on both uptake and production of dihydrogen. We, however, have to overcome the sensitivity against O2 of the enzyme, because hydrogenase i...
Hulin Tai Liyang Xu Koji Nishikawa Yoshiki Higuchi Shun Hirota
Chemical communications (Cambridge, England) 53(75) 10444-10447 2017年9月 [査読有り]
Previously, the Ni-SIr state of [NiFe] hydrogenase was found to convert to the Ni-SIa state by light irradiation. Herein, large activation energies and a large kinetic isotope effect were obtained for the reconversion of the Ni-SIa state to the Ni...
Biochimica et biophysica acta. Proteins and proteomics 1865(5) 481-487 2017年5月 [査読有り]
Electrostatic interactions between proteins are key factors that govern the association and reaction rate. We spectroscopically determine the second-order reaction rate constant (k) of electron transfer from [NiFe] hydrogenase (H2ase) to cytochrom...
INTERNATIONAL JOURNAL OF MICROGRAVITY SCIENCE AND APPLICATION 34(1) 2017年 [査読有り]
X-ray structure analysis method has been widely utilized to solve the structure of biological macromolecules. It is, however, also well known that the hydrogen atoms and protons, which are important to understand the structure-function relationshi...
Hong-qi Xia Keisei So Yuki Kitazumi Osamu Shirai Koji Nishikawa Yoshiki Higuchi Kenji Kano
JOURNAL OF POWER SOURCES 335 105-112 2016年12月 [査読有り]
A membraneless direct electron transfer (DET)-type dihydrogen (H-2)/air-breathing biofuel cell without any mediator was constructed wherein bilirubin oxidase from Myrothecium verrucaria (BOD) and membrane-bound [NiFe] hydrogenase from Desulfovibri...
大学本部
理学研究科