Hulin Tai Liyang Xu Seiya Inoue Koji Nishikawa Yoshiki Higuchi Shun Hirota
Physical chemistry chemical physics : PCCP 18(32) 22025-30 2016年8月 [査読有り]
The Ni-SIr state of [NiFe] hydrogenase from Desulfovibrio vulgaris Miyazaki F was photoactivated to its Ni-SIa state by Ar(+) laser irradiation at 514.5 nm, whereas the Ni-SL state was light induced from a newly identified state, which was less ac...
Keisei So Yuki Kitazumi Osamu Shirai Koji Nishikawa Yoshiki Higuchi Kenji Kano
JOURNAL OF MATERIALS CHEMISTRY A 4(22) 8742-8749 2016年6月 [査読有り]
H-2/O-2 biofuel cells utilizing hydrogenases and multicopper oxidases as bioelectrocatalysts are clean, sustainable, and environmentally friendly power devices. In this study, we constructed a novel gas diffusion bioelectrode with a sheet of water...
The purification procedure of Hyd-2-type [NiFe]-hydrogenase from Citrobacter sp. S-77 was improved by applying treatment with trypsin before chromatography. Purified protein samples both with and without trypsin treatment were successfully crystal...
Hulin Tai Koji Nishikawa Seiya Inoue Yoshiki Higuchi Shun Hirota
The journal of physical chemistry. B 119(43) 13668-74 2015年10月 [査読有り]
Different light-induced Ni-L states of [NiFe] hydrogenase from its Ni-C state have previously been observed by EPR spectroscopy. Herein, we succeeded in detecting simultaneously two Ni-L states of [NiFe] hydrogenase from Desulfovibrio vulgaris Miy...
The enzyme hydrogenase reversibly converts dihydrogen to protons and electrons at a metal catalyst. The location of the abundant hydrogens is of key importance for understanding structure and function of the protein. However, in protein X-ray crys...
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理学研究科