BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS 1865(5) 481-487 2017年5月 [査読有り]
Electrostatic interactions between proteins are key factors that govern the association and reaction rate. We spectroscopically determine the second-order reaction rate constant (k) of electron transfer from [NiFe] hydrogenase (H(2)ase) to cytochr...
The number of artificial protein supramolecules has been increasing; however, control of protein oligomer formation remains challenging. Cytochrome c from Allochromatium vinosum (AVCP) is a homodimeric protein in its native form, where its protome...
JOURNAL OF POWER SOURCES 335 105-112 2016年12月 [査読有り]
A membraneless direct electron transfer (DET)-type dihydrogen (H-2)/air-breathing biofuel cell without any mediator was constructed wherein bilirubin oxidase from Myrothecium verrucaria (BOD) and membrane-bound [NiFe] hydrogenase from Desulfovibri...
JOURNAL OF BIOCHEMISTRY 160(5) 251-258 2016年11月 [査読有り]
Hydrogenases catalyze the reversible conversion of molecular hydrogen to protons and electrons via a heterolytic splitting mechanism. The active sites of [NiFe] hydrogenases comprise a dinuclear Ni-Fe center carrying CO and CN- ligands. The cataly...