Chunmao He   Hideaki Ogata   Wolfgang Lubitz   
CHEMICAL SCIENCE 7(8) 5332-5340 2016年 [査読有り]
Nitrophorins (NPs) catalyze the nitrite dismutation reaction that is unprecedented in ferriheme proteins. Despite progress in studying the reaction mechanism, fundamental issues regarding the correlation of the structural features with the nitrite...
Hideaki Ogata   Tobias Kraemer   Hongxin Wang   David Schilter   Vladimir Pelmenschikov   Maurice van Gastel   Frank Neese   Thomas B. Rauchfuss   Leland B. Gee   Aubrey D. Scott   Yoshitaka Yoda   Yoshihito Tanaka   Wolfgang Lubitz   Stephen P. Cramer   
NATURE COMMUNICATIONS 6 2015年8月 [査読有り]
The metabolism of many anaerobes relies on [NiFe]-hydrogenases, whose characterization when bound to substrates has proven non-trivial. Presented here is direct evidence for a hydride bridge in the active site of the Fe-57-labelled fully reduced N...
The enzyme hydrogenase reversibly converts dihydrogen to protons and electrons at a metal catalyst(1). The location of the abundant hydrogens is of key importance for understanding structure and function of the protein(2-6). However, in protein X-...