Bacteriorhodopsin (bR) is a light-driven proton pump and a model membrane transport protein. We used time-resolved serial femtosecond crystallography at an x-ray free electron laser to visualize conformational changes in bR from nanoseconds to mil...
JOURNAL OF PHYSICAL CHEMISTRY B 120(32) 7836-7843 2016年8月 [査読有り]
CooA is a CO-sensing transcriptional activator from the photosynthetic bacterium Rhodospirillum rubrum that binds CO at the heme iron. The heme iron in ferrous CooA has two axial ligands: His77 and Pro2. CO displaces Pro2 and induces a conformatio...
Przemyslaw Nogly   Valerie Panneels   Garrett Nelson   Cornelius Gati   Tetsunari Kimura   Christopher Milne   Despina Milathianaki   Minoru Kubo   Wenting Wu   Chelsie Conrad   Jesse Coe   Richard Bean   Yun Zhao   Petra Bath   Robert Dods   Rajiv Harimoorthy   Kenneth R. Beyerlein   Jan Rheinberger   Daniel James   Daniel DePonte   Chufeng Li   Leonardo Sala   Garth J. Williams   Mark S. Hunter   Jason E. Koglin   Peter Berntsen   Eriko Nango   So Iwata   Henry N. Chapman   Petra Fromme   Matthias Frank   Rafael Abela   Sebastien Boutet   Anton Barty   Thomas A. White   Uwe Weierstall   John Spence   Richard Neutze   Gebhard Schertler   Jorg Standfuss   
NATURE COMMUNICATIONS 7 12314 2016年8月 [査読有り]
Serial femtosecond crystallography (SFX) using X-ray free-electron laser sources is an emerging method with considerable potential for time-resolved pump-probe experiments. Here we present a lipidic cubic phase SFX structure of the light-driven pr...
JOURNAL OF SYNCHROTRON RADIATION 23 334-338 2016年1月 [査読有り]
UV-visible absorption spectroscopy is useful for probing the electronic and structural changes of protein active sites, and thus the on-line combination of X-ray diffraction and spectroscopic analysis is increasingly being applied. Herein, a novel...
JOURNAL OF BIOLOGICAL CHEMISTRY 288(42) 30259-30269 2013年10月 [査読有り]
Background: Cytochrome c oxidase reduces O-2 coupled with proton pumping. Results: A newly developed time-resolved infrared system reveals transient conformational changes in the proton-pumping pathway upon CO binding to Cu-B in the O-2 reduction ...
Structural information on protein dynamics is a critical factor in fully understanding the protein functions. Pump-probe time-resolved serial femtosecond crystallography (TR-SFX) is a recently established technique for visualizing the structural c...