tRNAの量的調節機構の解析
,核-細胞質間物質輸送 tRNAの細胞内動態 tRNAのスプライシング RNAの品質管理
,nuclear-cytoplasmic transport intracellular dynamics of tRNA tRNA splicing quality control of RNA
lntrons are found in various tRNA genes in all the three kingdoms of life. Especially, archaeal and eukaryotic genomes are good sources of tRNA introns that are removed by proteinaceous splicing machinery. Most intron-containing tRNA genes both in...
Jiyao Song   Yasushi Tamura   Tohru Yoshihisa   Toshiya Endo   
EMBO REPORTS 15(6) 670-677 2014年6月 [査読有り]
The membrane topology of Om45 in the yeast mitochondrial outer membrane ( OM) is under debate. Here, we confirm that Om45 is anchored to the OM from the intermembrane space (IMS) by its N-terminal hydrophobic segment. We show that import of Om45 r...
Kayo Nozawa   Ryuichiro Ishitani   Tohru Yoshihisa   Mamoru Sato   Fumio Arisaka   Shuji Kanamaru   Naoshi Dohmae   Dev Mangroo   Bruno Senger   Hubert D. Becker   Osamu Nureki   
NUCLEIC ACIDS RESEARCH 41(6) 3901-3914 2013年4月 [査読有り]
In all eukaryotes, transcribed precursor tRNAs are maturated by processing and modification processes in nucleus and are transported to the cytoplasm. The cytoplasmic export protein (Cex1p) captures mature tRNAs from the nuclear export receptor (L...
Shunsuke Mori   Takuya Kajita   Toshiya Endo   Tohru Yoshihisa   
RNA-A PUBLICATION OF THE RNA SOCIETY 17(9) 1760-1769 2011年9月 [査読有り]
A part of eukaryotic tRNA genes harbor an intron at one nucleotide 3' to the anticodon, so that removal of the intron is an essential processing step for tRNA maturation. While some tRNA introns have important roles in modification of certain...