tRNAの量的調節機構の解析
,核-細胞質間物質輸送 tRNAの細胞内動態 tRNAのスプライシング RNAの品質管理
,nuclear-cytoplasmic transport intracellular dynamics of tRNA tRNA splicing quality control of RNA
T Taura   T Yoshihisa   K Ito   
BIOCHIMIE 79(8) 517-521 1997年9月 [査読有り]
Protein translocation across the plasma membrane of E coli is facilitated by Sec factors, including the membrane-embedded SecYEG subunit and the SecA ATPase. Although there is complete agreement that SecA is essential for protein translocation, so...
T Homma   T Yoshihisa   K Ito   
FEBS LETTERS 408(1) 11-15 1997年5月 [査読有り]
We used hexahistidine-tagged SecE and SecY to study how the core subunits (SecY, SecE and SecG) of Escherichia coli protein translocase interact with each other. Detergent extracts were prepared from the plasma membranes and fractionated by Ni2+-N...
JOURNAL OF BIOLOGICAL CHEMISTRY 271(16) 9429-9436 1996年4月
We examined in vitro translocation of pro-OmpA derivatives with a His(6) tag at various positions in their mature proteins and with a c-Myc tag at their C termini across inverted membrane vesicles of Escherchia coil, Those with a His(6) tag in the...
T HOMMA   T YOSHIHISA   A KIHARA   Y AKIYAMA   K ITO   
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 215(2) 452-458 1995年10月 [査読有り]
Intracellular stability of alpha fragments of beta-galactosidase in Escherichia coli has been studied by pulse-chase/immunoprecipitation experiments. An alpha fragment encoded by the pUC118 vector was relatively stable with an estimated half-life ...