The enzymatic recycling of polyethylene terephthalate (PET) can be a promising approach to tackle the problem of plastic waste. The thermostability and activity of PET-hydrolyzing enzymes are still insufficient for practical application....
Tadeo Moreno-Chicano   Leiah M Carey   Danny Axford   John H Beale   R Bruce Doak   Helen M E Duyvesteyn   Ali Ebrahim   Robert W Henning   Diana C F Monteiro   Dean A Myles   Shigeki Owada   Darren A Sherrell   Megan L Straw   Vukica Šrajer   Hiroshi Sugimoto   Kensuke Tono   Takehiko Tosha   Ivo Tews   Martin Trebbin   Richard W Strange   Kevin L Weiss   Jonathan A R Worrall   Flora Meilleur   Robin L Owen   Reza A Ghiladi   Michael A Hough   
IUCrJ 9(Pt 5) 610-624 2022年9月
Room-temperature macromolecular crystallography allows protein structures to be determined under close-to-physiological conditions, permits dynamic freedom in protein motions and enables time-resolved studies. In the case of metalloenzymes that ar...
Samuel L Rose   Seiki Baba   Hideo Okumura   Svetlana V Antonyuk   Daisuke Sasaki   Tobias M Hedison   Muralidharan Shanmugam   Derren J Heyes   Nigel S Scrutton   Takashi Kumasaka   Takehiko Tosha   Robert R Eady   Masaki Yamamoto   S Samar Hasnain   
Proceedings of the National Academy of Sciences of the United States of America 119(30) e2205664119 2022年7月
Many enzymes utilize redox-coupled centers for performing catalysis where these centers are used to control and regulate the transfer of electrons required for catalysis, whose untimely delivery can lead to a state incapable of binding the substra...
Proceedings of the National Academy of Sciences of the United States of America 119(27) e2123385119 2022年7月
Bacterial pathogens acquire heme from the host hemoglobin as an iron nutrient for their virulence and proliferation in blood. Concurrently, they encounter cytotoxic-free heme that escapes the heme-acquisition process. To overcome this toxicity, ma...
Hirotoshi Matsumura   Abayomi S Faponle   Peter-Leon Hagedoorn   Takehiko Tosha   Sam P de Visser   Pierre Moënne-Loccoz   
Journal of inorganic biochemistry 231 111781-111781 2022年6月
Steady-state kinetics of cytochrome-c dependent denitrifying NO reductases (cNORs) show evidence of substrate inhibition at NO concentrations higher than 10 μM, but the mechanism of inhibition remains unclear. Here, we present low-temperature FTIR...
BIN MOHAMAD JAMALI Muhamad Arif   GOPALASINGAM Chai C.   JOHNSON Rachel M.   MUENCH Stephen P   ANTONYUK Svetlana V.   HASNAIN Samar S.   TOSHA Takehiko   MURAMOTO Kazumasa   SHIRO Yoshitsugu