柴田直樹

研究者氏名	柴田直樹
	シバタナオキ
URL	http://www.sci.u-hyogo.ac.jp/life/biophys1/index-j.html
所属	兵庫県立大学
部署	大学院理学研究科生命科学専攻
職名	助教授・准教授
学位	博士(工学)(大阪大学)
J-Global ID	200901039667162990

研究キーワード

結晶化 ,構造生物学 ,タンパク質 ,Ｘ線結晶構造解析 ,protein crystallization ,structural studies ,X-ray crystallography

研究分野

ライフサイエンス / 構造生物化学 /

受賞

2003年12月

日本結晶学会, 平成15年度日本結晶学会賞進歩賞
柴田直樹

論文

<2 345 6 >

Structural Changes of the Trinuclear Copper Center in Bilirubin Oxidase upon Reduction.

Takaki Tokiwa Mitsuo Shoji Vladimir Sladek Naoki Shibata Yoshiki Higuchi Kunishige Kataoka Takeshi Sakurai Yasuteru Shigeta Fuminori Misaizu

Molecules (Basel, Switzerland) 24(1) 2018年12月 [査読有り]

Geometric and electronic structure changes in the copper (Cu) centers in bilirubin oxidase (BOD) upon a four-electron reduction were investigated by quantum mechanics/molecular mechanics (QM/MM) calculations. For the QM region, the unrestricted de...

Redox Potential-Dependent Formation of an Unusual His-Trp Bond in Bilirubin Oxidase.

Mahfuza Akter Takaki Tokiwa Mitsuo Shoji Koji Nishikawa Yasuteru Shigeta Takeshi Sakurai Yoshiki Higuchi Kunishige Kataoka Naoki Shibata

Chemistry (Weinheim an der Bergstrasse, Germany) 24(68) 18052-18058 2018年12月 [査読有り]

Bilirubin oxidase (BOD) belongs to the family of blue multicopper oxidases, and catalyzes the concomitant oxidation of bilirubin to biliverdin and the reduction of molecular oxygen to water via a four-electron reduction system. The active sites of...

Structural basis of the correct subunit assembly, aggregation, and intracellular degradation of nylon hydrolase.

Seiji Negoro Naoki Shibata Young-Ho Lee Ikki Takehara Ryo Kinugasa Keisuke Nagai Yusuke Tanaka Dai-Ichiro Kato Masahiro Takeo Yuji Goto Yoshiki Higuchi

Scientific reports 8(1) 9725-9725 2018年6月 [査読有り]

Nylon hydrolase (NylC) is initially expressed as an inactive precursor (36 kDa). The precursor is cleaved autocatalytically at Asn266/Thr267 to generate an active enzyme composed of an α subunit (27 kDa) and a β subunit (9 kDa). Four αβ heterodime...

Direct Participation of a Peripheral Side Chain of a Corrin Ring in Coenzyme B₁₂ Catalysis.

Shibata N Sueyoshi Y Higuchi Y Toraya T

Angewandte Chemie (International ed. in English) 57(26) 7830-7835 2018年6月 [査読有り]

Construction of a Triangle-Shaped Trimer and a Tetrahedron Using an α-Helix-Inserted Circular Permutant of Cytochrome c₅₅₅.

Oda A Nagao S Yamanaka M Ueda I Watanabe H Uchihashi T Shibata N Higuchi Y Hirota S

Chemistry, an Asian journal 13(8) 964-967 2018年4月 [査読有り]

MISC

12 3 4 5 >

O2により不活性化されたラジカルB12酵素ジオールデヒドラターゼの構造解析と補酵素分解物の同定

柴田直樹樋口芳樹虎谷哲夫

第468回ビタミンB研究協議会 2022年9月

ナイロン分解酵素NylB の構造進化，触媒機構とアミド合成への応用

根来誠司武尾正弘柴田直樹樋口芳樹加藤太一郎重田育照

月刊バイオインダストリー 2019年6月 [招待有り]

Formation and carbon monoxide-dependent dissociation of Allochromatium vinosum cytochrome c' oligomers using domain-swapped dimers

Yamanaka Masaru Hoshizumi Makoto Nagao Satoshi Nakayama Ryoko Shibata Naoki Higuchi Yoshiki Hirota Shun

2017年2月

The number of artificial protein supramolecules has been increasing; however, control of protein oligomer formation remains challenging. Cytochrome c′ from Allochromatium vinosum (AVCP) is a homodimeric protein in its native form, where its protom...

3P-046 ナイロン加水分解酵素のサブユニット界面でのアミノ酸置換と熱安定化効果(酵素学,酵素工学,一般講演)

衣笠凌羽田圭吾谷本悠樹竹原一起柴田直樹加藤太一郎武尾正弘根来誠司

日本生物工学会大会講演要旨集 67 282-282 2015年

Preparation of single crystals of Multicopper oxidases for neutron diffraction study

Y. Higuchi M. Akter C. Inoue Y. Shomura N. Shibata K. Inaka K. Kataoka T. Sakurai K. Komori

JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY 19 S324-S324 2014年3月