Multicopper oxidases oxidize various phenolic and nonphenolic compounds by using molecular oxygen as an electron acceptor to produce water. A multicopper oxidase protein, CueO, from Escherichia coli is involved in copper homeostasis in the bacteri...
Seiji Negoro Yasuyuki Kawashima Naoki Shibata Tatsuya Kobayashi Takeshi Baba Young-Ho Lee Katsumasa Kamiya Yasuteru Shigeta Keisuke Nagai Ikki Takehara Dai-ichiro Kato Masahiro Takeo Yoshiki Higuchi
FEBS LETTERS 590(18) 3133-3143 2016年9月 [査読有り]
The enzyme 6-aminohexanoate-dimer hydrolase catalyzes amide synthesis. The yield of this reverse reaction in 90% t-butyl alcohol was found to vary drastically when enzyme mutants with substitutions of several amino acids located at the entrance of...
JOURNAL OF BIOCHEMISTRY 158(4) 271-292 2015年10月 [査読有り][招待有り]
Certain proteins utilize the high reactivity of radicals for catalysing chemically challenging reactions. These proteins contain or form a radical and therefore named 'radical enzymes'. Radicals are introduced by enzymes themselves or by (re)activ...
NAD(+)-reducing [NiFe] hydrogenases catalyze the oxidoreduction of dihydrogen concomitant with the interconversion of NAD(+) and NADH. Here, the isolation, purification and crystallization of the NAD(+)-reducing [NiFe] hydrogenase from Hydrogenoph...
The number of artificial protein supramolecules has been increasing; however, control of protein oligomer formation remains challenging. Cytochrome c′ from Allochromatium vinosum (AVCP) is a homodimeric protein in its native form, where its protom...
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