Atsuhiro Shimada Minoru Kubo Seiki Baba Keitaro Yamashita Kunio Hirata Go Ueno Takashi Nomura Tetsunari Kimura Kyoko Shinzawa-Itoh Junpei Baba Keita Hatano Yuki Eto Akari Miyamoto Hironori Murakami Takashi Kumasaka Shigeki Owada Kensuke Tono Makina Yabashi Yoshihiro Yamaguchi Sachiko Yanagisawa Miyuki Sakaguchi Takashi Ogura Ryo Komiya Jiwang Yan Eiki Yamashita Masaki Yamamoto Hideo Ago Shinya Yoshikawa Tomitake Tsukihara
SCIENCE ADVANCES 3(7) e1603042 2017年7月
Bovine cytochrome c oxidase (CcO), a 420-kDa membrane protein, pumps protons using electrostatic repulsion between protons transferred through a water channel and net positive charges created by oxidation of heme a (Fe-a) for reduction of O-2 at h...
JOURNAL OF RAMAN SPECTROSCOPY 48(5) 680-685 2017年5月
An improved stopped-flow resonance Raman spectroscopy device was constructed using a stopped-flow mixer with a dead time of 3ms and a mixing volume of 0.1mL. The device was tested using myoglobin, where the formation reaction of a high-valent heme...
JOURNAL OF BIOLOGICAL CHEMISTRY 291(8) 4178-4184 2016年2月
To understand the roles of mitochondrial respiratory chain supercomplexes, methods for consistently separating and preparing supercomplexes must be established. To this end, we solubilized supercomplexes from bovine heart mitochondria with digiton...
We analyzed the oxygen (O-2) and carbon monoxide (CO) binding properties, autoxidation reaction rate, and FeO2 and FeCO vibrational frequencies of the H64Q mutant of sperm whale myoglobin (Mb) reconstituted with chemically modified heme cofactors ...
JOURNAL OF SYNCHROTRON RADIATION 23 334-338 2016年1月
UV-visible absorption spectroscopy is useful for probing the electronic and structural changes of protein active sites, and thus the on-line combination of X-ray diffraction and spectroscopic analysis is increasingly being applied. Herein, a novel...
An improved stopped-flow resonance Raman spectroscopy device was constructed using a stopped-flow mixer with a dead time of 3 ms and a mixing volume of 0.1 mL. The device was tested using myoglobin, where the formation reaction of a high-valent he...
大学本部
理学研究科