JOURNAL OF BIOSCIENCE AND BIOENGINEERING 126(3) 355-362 2018年9月 [査読有り]
3-Methyl-4-nitrophenol (3M4NP) is formed in soil as a hydrolysis product of fenitrothion, one of the major organophosphorus pesticides. A Pseudomonas strain was isolated as a 3M4NP degrader from a crop soil and designated TSN1. This strain utilize...
Nylon hydrolase (NylC) is initially expressed as an inactive precursor (36 kDa). The precursor is cleaved autocatalytically at Asn266/Thr267 to generate an active enzyme composed of an a subunit (27 kDa) and a beta subunit (9 kDa). Four alpha beta...
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 102(2) 801-814 2018年1月 [査読有り]
Arthrobacter sp. strain KI72 grows on a 6-aminohexanoate oligomer, which is a by-product of nylon-6 manufacturing, as a sole source of carbon and nitrogen. We cloned the two genes, nylD (1) and nylE (1) , responsible for 6-aminohexanoate metabolis...
Juri Maeda   Dai-ichiro Kato   Masatoshi Okuda   Masahiro Takeo   Seiji Negoro   Kazunari Arima   Yuji Ito   Kazuki Niwa   
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 1861(8) 2112-2118 2017年8月 [査読有り]
Due to the strict enantioselectivity of firefly luciferase, only D-luciferin can be used as a substrate for bioluminescence reactions. Unfortunately, luciferin racemizes easily and accumulation of nonluminous L-luciferin has negative influences on...