A simple reaction procedure for chemiluminescence of firefly luciferin (D-luc) using n-propylphosphonic anhydride (T3P) is reported. A luminescent photon is produced as a result of one-pot reaction, only requiring mixing with the substrate carboxy...
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 97(21) 9569-9577 2013年11月 [査読有り]
Some strains belonging to the genera Citrobacter and Enterobacter have been reported to produce chitin/chitosan-like bioflocculants (BFs) from acetate. In this study, to investigate the distribution of the BF-producing potential in the genus Citro...
Journal of biotechnology 168(3) 277-83 2013年11月 [査読有り]
We identified the critical amino acid residues for substrate recognition using two firefly luciferases from Pylocoeria miyako (PmL) and Hotaria parvura (HpL), as these two luciferase enzymes exhibit different activities toward ketoprofen. Specific...
Nylon hydrolase (NylC) encoded by Arthrobacter plasmid pOAD2 (NylC(p2)) was expressed in Escherichia coli JM109 and purified by ammonium sulfate fractionation, anion-exchange column chromatography and gel-filtration chromatography. NylC(p2) was cr...