Sphingomonas sp. NP5 can degrade a wide range of nonylphenol (NP) isomers that have widely contaminated aquatic environments as major endocrine-disrupting chemicals. To understand the biochemical and genetic backgrounds of NP degradation, a gene l...
JOURNAL OF BIOLOGICAL CHEMISTRY 287(7) 5079-5090 2012年2月 [査読有り]
We performed x-ray crystallographic analyses of the 6-aminohexanoate oligomer hydrolase (NylC) from Agromyces sp. at 2.0 angstrom-resolution. This enzyme is a member of the N-terminal nucleophile hydrolase superfamily that is responsible for the d...
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 75(9) 1758-1762 2011年9月 [査読有り]
Measurement of thioesterification activities for dodecanoic acid (C12) and ketoprofen was done using five firefly luciferases, from Pyrocoelia miyako (PmL), Photinus pyralis (PpL), Luciola cruciata (LcL), Hotaria parvura (HpL), and Luciola mingrel...
6-Aminohexanoate-oligomer hydrolase (NylC) from Agromyces sp. KY5R was expressed in Escherichia coli JM109 and purified by ammonium sulfate fractionation, anion-exchange column chromatography and gel-filtration chromatography. NylC was crystallize...
JOURNAL OF BIOSCIENCE AND BIOENGINEERING 111(6) 687-694 2011年6月 [査読有り]
4-Nitrophenol (4-NP) is a toxic compound formed in soil by the hydrolysis of organophosphorous pesticides, such as parathion. We previously reported the presence of the 4-NP degradation gene cluster (nphRA1A2) in Rhodococcus sp. strain PN1, which ...
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