BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 71(12) 3098-3101 2007年12月 [査読有り]
Microfluid filters were fabricated, which possessed 2,100 cylindrical through-bores (phi 40 mu m) in 200 mu m-thickness polymethylmethacrylate (PMMA) sheets (phi 3 mm), by deep X-ray lithography using synchrotron radiation. To evaluate the microfl...
JOURNAL OF BIOSCIENCE AND BIOENGINEERING 104(6) 521-524 2007年12月 [査読有り]
A 15-kb gene locus including nylon-oligomer-degrading genes from the chromosome of an alkalophilic bacterium, Agromyces sp. KY5R, was cloned and sequenced. The genetic organization was similar to the DNA region flanked by directly repeated IS6100 ...
APPLIED AND ENVIRONMENTAL MICROBIOLOGY 73(21) 7099-7102 2007年11月 [査読有り]
Alkalophilic, nylon oligomer-degrading strains, Agromyces sp. and Kocuria sp., were isolated from the wastewater of a nylon-6 factory and from activated sludge from a sewage disposal plant. The 6-aminohexanoate oligomer hydrolases (Ny1C) from the ...
JOURNAL OF BIOSCIENCE AND BIOENGINEERING 104(4) 309-314 2007年10月 [査読有り]
Alkylcatechol 2,3-dioxygenase was purified from the cell extract of recombinant Escherichia coli JM109 harboring the alkylcatechol 2,3-dioxygenase gene (bupB) cloned from the butylphenol-degrading bacterium Pseudomonas putida MT4. The purified enz...
JOURNAL OF BIOSCIENCE AND BIOENGINEERING 104(4) 347-350 2007年10月 [査読有り]
We developed an enzyme-linked immunosorbent assay for an endocrine disrupter, nonylphenol, using a microreactor composed of two reaction vessels stacked vertically through a microfluid filter. The filters constructed by deep X-ray lithography poss...