We introduce a new application of firefly luciferase (EC 1.13.12.7). The firefly luciferases belong to a large superfamily that includes rat liver long-chain acyl-CoA syntbetase (LACS1). LACS1 is the enzyme that is involved in the deracernization ...
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 71(7) 1668-1675 2007年7月 [査読有り]
Catechol 2,3-dioxygenase (C23O), a key enzyme in the meta-cleavage pathway of catechol metabolism, was purified from cell extract of recombinant Escherichia coli JM109 harboring the C23O gene (atdB) cloned from an aniline-degrading bacterium Acine...
JOURNAL OF MOLECULAR BIOLOGY 370(1) 142-156 2007年6月 [査読有り]
We performed X-ray crystallographic analyses of 6-aminohexanoate-dimer hydrolase (Hyb-24DN), an enzyme responsible for the degradation of nylon-6, an industry by-product, and of a complex between Hyb-24DNA- 12 (S112A-mutant of Hyb-24DN) and 6-amin...
6-Aminohexanoate-cyclic-dimer hydrolase (EI) from Arthrobacter sp. KI72 was expressed in Escherichia coli and purified by anion-exchange chromatography. EI was crystallized by the sitting-drop vapour-diffusion method with sodium citrate as precipi...
Masahiro Takeo   Subbuswamy K. Prabu   Chitoshi Kitamura   Makoto Hirai   Hana Takahashi   Dai-ichiro Kato   Seiji Negoro   
JOURNAL OF BIOSCIENCE AND BIOENGINEERING 102(4) 352-361 2006年10月 [査読有り]
Alkylphenols (APs) are ubiquitous contaminants in aquatic environments and have endocrine disrupting and toxic effects on aquatic organisms. To investigate biodegradation mechanisms of APs, an AP degradation gene cluster was cloned from a butylphe...