Xenopus laevis is a widely used model organism in developmental and regeneration studies. Despite several reports regarding targeted integration techniques in Xenopus, there is still room for improvement of them, especially in creating reporter li...
Transgenic techniques have greatly increased our understanding of the transcriptional regulation of target genes through live reporter imaging, as well as the spatiotemporal function of a gene using loss- and gain-of-function constructs. In Xenopu...
The coincidence of cell cycle exit and differentiation has been described in a wide variety of stem cells and organisms for decades, but the causal relationship is still unclear due to the complicated regulation of the cell cycle. Here, we used th...
Development (Cambridge, England) 149(3) 2022年2月 [査読有り]
The regenerative ability of planarians relies on their adult pluripotent stem cell population. Although all stem cells express a piwi homolog, recently it has become possible to classify the piwi+ stem cell population into specialized subpopulatio...
Planarians belong to the phylum Platyhelminthes and can regenerate their missing body parts after injury via activation of somatic pluripotent stem cells called neoblasts. Previous studies suggested that fibroblast growth factor (FGF) signaling pl...
The study of planarian regeneration may help us to understand how we can rebuild organs and tissues after injury, disease or ageing(1). The robust regenerative abilities of planarians are based upon a population of totipotent stem cells (neoblasts...
S Koinuma   Y Umesono   K Watanabe   K Agata   
GENE 259(1-2) 171-176 2000年12月
We have isolated a planarian Forkhead box A (FoxA, a new name for a gene group containing HNF3 alpha,beta,gamma)-related gene, DjFoxA, and examined its spatial and temporal distribution in both intact and regenerating planarians by in situ hybridi...
In the freshwater planarian Dugesia japonica, five cDNAs for HOM/HOX homeobox genes were cloned and sequenced. Together with sequence data on MOM/HOX homeobox genes of platyhelminthes deposited in databases, comparison of the deduced amino acid se...